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    • Gatalyst™ Donor
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  • Gatalyst™ Donor
  • Gatalyst™ CRISPR
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Gatalyst™ CRISPR Cell line GENERATION

Stellate DNA is the leading provider of custom genome editing services. Leverage our experts and optimized CRISPR/Cas9 cell line modeling service platform Gatalyst™ CRISPR for hassle-free research. Gatalyst™ CRISPR enables large fragments excision or correct mutations in various cell lines.

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Cell line generation service overview

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Advantages

Why work with Stellate DNA?

Advantages

  • Knock-in is defined as an exogenous gene inserted in a native locus of interest. A double-stranded break is introduced by CRISPR-Cas9 to a specific site in the genome. Via homology-directed repair (HDR) pathway, the circular single stranded Gatalyst™ donor sequence alone with homologous arms can be introduced into that locus. Gatalyst™ CR

  • Knock-in is defined as an exogenous gene inserted in a native locus of interest. A double-stranded break is introduced by CRISPR-Cas9 to a specific site in the genome. Via homology-directed repair (HDR) pathway, the circular single stranded Gatalyst™ donor sequence alone with homologous arms can be introduced into that locus. Gatalyst™ CRISPR technology enables large fragment up to 20 kb knock-ins in variety of mammalian cell lines.

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Cell DNA sequence

Workflow

Why work with Stellate DNA?

Advantages

Host cell line prep -> Gatalyst™ circular single strand donor prep ->Transfection of host cells -> Single cell clone generation -> Positive clone screening ->  Clone cryopreservation and QC  


Stellate DNA’s Gatalyst™ CRISPR cell line modeling services enable large fragment excision or accurate mutation(s) – providing knockin and knockout cell lines with complete edited effect of the target gene.

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Why work with Stellate DNA?

Why work with Stellate DNA?

Why work with Stellate DNA?

  • Guaranteed: 100% customer satisfaction guaranteed
  • Substantial experience: Experience with thousands of gene editing projects delivered
  • Optimized technology: By using optimized CRISPR/Cas9 technology Gatalyst™ CRISPR, stable expression of knock-in gene can be achieved

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