Gatalyst™ CRISPR Cell line GENERATION
Stellate DNA is the leading provider of custom genome editing services. Leverage our experts and optimized CRISPR/Cas9 cell line modeling service platform Gatalyst™ CRISPR for hassle-free research. Gatalyst™ CRISPR enables large fragments excision or correct mutations in various cell lines.
Cell line generation service overview
Advantages
Why work with Stellate DNA?
Advantages
- Knock-in is defined as an exogenous gene inserted in a native locus of interest. A double-stranded break is introduced by CRISPR-Cas9 to a specific site in the genome. Via homology-directed repair (HDR) pathway, the circular single stranded Gatalyst™ donor sequence alone with homologous arms can be introduced into that locus. Gatalyst™ CR
- Knock-in is defined as an exogenous gene inserted in a native locus of interest. A double-stranded break is introduced by CRISPR-Cas9 to a specific site in the genome. Via homology-directed repair (HDR) pathway, the circular single stranded Gatalyst™ donor sequence alone with homologous arms can be introduced into that locus. Gatalyst™ CRISPR technology enables large fragment up to 20 kb knock-ins in variety of mammalian cell lines.
Workflow
Why work with Stellate DNA?
Advantages
Host cell line prep -> Gatalyst™ circular single strand donor prep ->Transfection of host cells -> Single cell clone generation -> Positive clone screening -> Clone cryopreservation and QC
Stellate DNA’s Gatalyst™ CRISPR cell line modeling services enable large fragment excision or accurate mutation(s) – providing knockin and knockout cell lines with complete edited effect of the target gene.
Why work with Stellate DNA?
Why work with Stellate DNA?
Why work with Stellate DNA?
- Guaranteed: 100% customer satisfaction guaranteed
- Substantial experience: Experience with thousands of gene editing projects delivered
- Optimized technology: By using optimized CRISPR/Cas9 technology Gatalyst™ CRISPR, stable expression of knock-in gene can be achieved